PURIFICATION OF THE
ADHESIN FROM BIFIDOBACTERIUM
ZHENG Y-J1, PAN L-J2, WANG L-S2 & ZHOU D-Y2
1 Shenzhen Children’s Hospital,
Shenzhen, China
2 Institute of Gastroenterology of
PLA, First Military Medical University, Guangzhou, China
Objective: To determine
the adhesin from bifidobacterium.
Methods: The Bifidobacterium adolescetis was
tested for its adherence to human intestinal cell line Lovo cells. The
adhesin of bifidobacterium was isolated and purified by ammonium sulfate
precipitation, superdex 75 gel filtration and Q-Sepharose FF ion exchange
chromatography, and the adhesin was analyzed by SDS-PAGE.
Results: The Bifidobacterium adolescetis cultured
in agar and the bateria in THIO resuspended with spent culture supernatant
expressed the same adherence capacities to Lovo cells. Pre-treatment of the
spent culture supernatant with trypsin or 100℃ resulted in a marked impairment of adherence. A 16kDa protein
was purified and identified as the adhesin.
Conclusions: The Bifidobacterium adolescentis
possesses both cell surface-associated and soluble adhesin which presents
in the spent culture supernatant. The soluble adhesin may be a
heat-sensitive 16kDa protein.