THE DETECTION OF
ANTI-PPDIgG AND TB-DNA IN CEREBROSPINAL FLUID OF THE PATIENTS WITH
TUBERCULOUS MENINGITIS
ZHENG Y-J1, PAN K-L2, QIAN X-H2
1 Shenzhen Children¡¯s Hospital, Shenzhen, China
2
Xijing Hospital, Fourth Military Medical University, Xian, China
Objective:
To explore the diagnostic value of the IgG
antibody to purified protein derivative (Anti-PPDIgG) and the tuberculosis
bacilli DNA (TB-DNA) in the children with tuberculous meningitis.
Methods: Cerebrospinal fluid (CSF) sample come from 27 children with
tuberculous meningitis and 10 children with non-tuberculous meningitis.
Anti-PPDIgG was detected by ELISA and TB-DNA detected by PCR. The CSF
culture was conducted at the same time.
Results:
The sensitivities of the Anti-PPDIgG, TB-DNA
and culture positive were 76.2%, 77.8% and 11.1% respectively; the
specificities were 80.0%, 100.0% and 100.0% respectively. The positive
predictive value of the Anti-PPDIgG and TB-DNA were 88.9% and 100.0% and
the negative predictive value were 61.2% and 62.5% respectively.
Conclusions: The TB-DNA by PCR and the Anti-PPDIgG by ELISA may be useful
for the diagnosis of the children with tuberculous meningitis. The TB-DNA
by PCR may be superior to the Anti-PPDIgG by ELISA.