文本框: APOLIPOPROTEIN DEPOSITION IN GLOMERULI IN CHILDREN NEPHRITIC SYNDROME Gao YF, Liu GL, Xia ZK, Fu J, F YF, Fan ZM, Zhang LF Department of Pediatric, Nanjing Jinling Hospital, Nanjing, China Objective: To investigate the relationship between apolipoprotein deposition in glomeruli and its clinical manifestation and pathological type in chidren nephritic syndrome. Methods: Immunocytochemical study was performed on 38 renal biopsy specimens, using monoclonal antibodies against human apolipoprotein (apo) A-I, apo B, apo E and polyclonal antibody against low density lipoprotein (LDL) receptor and scavenger receptor. Results: Apo B and Apo E deposition was mainly located in mesangium areas in MsPGN and MPGN, otherwise in MCNS. their immuno-fluorescence intensity and distribution correlation with mesanial cells proliferation and mesangial area extension. Apo B and Apo E were also stained in sclerosed areas of glomeruli. Apo A-I deposition was localized mainly in glomerular epithelial cells and the cytoplasm of tubular epithelial cells in MCNS, MsPGN, MPGN and FSGS, its weakly in mesangial areas and lost in sclerosed areas. In clinical, Apo B and Apo E deposition in glomeruli were highly associated with proteinuria and respondence to steroids. Conclusion: These results suggest that the deposition of Apo B and Apo E accelerates the progression of mesangial lesions, resulting in high degrees of proteinuria and glomerular sclerosis. 1000