THE EXPRESSION OF IL-10, IL-12 AND IFN-γ IN INTRACELLUAR AND
SERUM OF CHILDREN WITH ALL
Sheng GY, Li L, Liu YF, Zhou X, Fang YQ, Zhao XM
The First Affiliated Hospital of Henan Medical University,
Zhengzhou, China
Objective: To investigation the relationship between the cytokine network
represented by maladjustment of the 3 cytokines (CKs) (IL-12, IL-10 and
IFN-γ) and the pathogenic mechanism of leukemia.
Methods: The expression of IL-12, IL-10 and IFN-γ in mononuclear cell
of 7 children with acute T lymphoblastic leukemia (T-ALL) and 8 healthy
children were detected by flow cytometry (FCM) (the mononuclear cell were
stimulated for 5 hours); the expression of the 3 cytokines in serum were
detected by ELISA technique in 47 children with ALL and 20 healthy children
and the relation correlativity analysis was made.
Results: 1) IL-12 had positive expression in non-leukemic T cell (CD3+
CD7+) of 4 children with T-ALL, but no expression in
leukemic blast T cell (CD3- CD7+)
of children with T-ALL or in T cell (CD3+ CD7+)
of healthy children. 2) There was not IL-10 positive expression in
non-leukemic T cells and leukemic cells of children with T-All or in T
cells of healthy children. 3) IFN-γ had positive expression both in non-leukemic T cells of 3 children with
T-ALL and in normal T cells of 8 healthy children, but no expression in
leukemic cells of children with T-All. 4) The level of of IL-10 in ALL
children serum was higher than that in control group serum and the
difference was significant. 5) The level of IL-12 and IFN-γ in ALL children’s
serum were all lower than that in control group’s serum and the differences
were significant. 6) The serum levels of IL-12 and IFN-γ in children with ALL
was positively correlated, while those of IL-10 and IFN-γ, IL-10 and IL-12 were
negatively correlated.
Conclusions: 1) Intracelluar CKs can be detected in the leukemic cells from
children with T-ALL by multiparameter flow cytoimetry analysis afler cells
stimulated for 5 hours. 2) The maladjustment of cytokines can cause the
disorder of molecular metablism in blood cells and uncontrolable cell
multiplication, which may be one of the most significant pathogenic
mechanisms of leukemia.