Text Box: ANALYSIS OF EFFECTS OF PROLIFERATION APOPTOSIS AND NECROSIS ON HL-60 CELLS INDUCED BY CYTOCHROME C Chen R-L1, Chen M-Z1, Wang J-X2, et al Department of pediatrics, Affiliated Hospital, Guangdong Medical College, Zhanjiang, China Objective: To determine and analyze the effects of proliferation appoptosis and necrosis on HL-60 cells induced by different concentration of cytochrome C. Methods: HL-60 cells were stimulated with different concentration of cytochrome C. The effect of cytochrome C on HL-60 cells were determined by flow cytometry and eletrophoresis. The appropriate concentration and the interactive time of cytochrome C were also performed. Results: Different concentration of cytochrome C could induce effects of proliferation, apoptosis and necrosis on HL-60 cells. Concentration of cytochrome C in proliferation and necrosis was <0.94 ug/ml and >28.13 ug/ml, respectivly. And the appropriate concentration of cytochrome C in apoptosis was 9.38ug/ml-22.5 ug/ml. The appropriate interactive time was 24 hour. Conclusions: A certain concentration of cytochrome C can induce apoptosis of HL-60 cells. Being a kind of apoptosis inducers of lukemic cells, cytochrome C may be used widely in clinical therapy in the future. 1034