A STUDY ON SERUM LEVELS OF IL-4, IL-5, IL-10
AND INTERCELLULAR ADHESION MOLECULE-1 IN CHILDREN WITH RESPIRATORY
SYNCYTIAL VIRUS BRONCHIOLITIS
Dong L, Li CC, Cheng XF, et al.
Yuyin
Children’s Hospital Affiliated to Wenzhou Medical College, Wenzhou, China
Objective:
Respiratory syncytial virus （RSV）bronchiolitis is
associated with subsequent recurrent wheezing episode and asthma. To determine
a role of Th-2 type cytokines and intercellular adhesion molecule in airway
inflammations of RSV bronchiolitis. We measured Interleukin-4 (IL-4)、Interleukin-5
(IL-5), Interleukin-10 (IL-10) and soluble intercellular adhesion
molecule-1 (SICAM-1) concentrations in serum in children with RSV
bronchiolitis during acute phase and recovery one.
Methods:
Sera were collected from patients who were admitted to pediatric ward. The
diagnosis of RSV infection was based on alkalinephosphatase
antialkalinephosphatase (APAAP) bridge linked enzyme labelling assay and
ELISA in 41 cases. 37 healthy children were served as normal controls.
Levels of cytokines and SICAM-1 were analyzed by ELISA.
Results:
(1) Serum levels of IL-4, IL-5, IL-10 in children during acute phase of RSV bronchiolitis were significantly
higher than those of normal controls (all p<0.05). There was remarkable difference
of SICAM-1 level between two groups. (2) Levels of IL-4, IL-10 decreased significantly
during recovery phase of the disease, and had no differences as compared with
normal controls, while IL-5 value during recovery phase was still higher than those of normal
controls. Concentrations of SICAM-1 during recovery phase was lower than those
of acute phase and had no difference compared with normal controls.
Conclusions: There is a predominant
expression of Th-2 type cytokines and increased SICAM-1 expression in children
with RSV bronchiolitis. This may contribute to airway inflammation and hyperresponsiveness
during RSV infection.