1080

A STUDY ON CORD BLOOD CIK CELLS – ANTITUMOR ACTIVITY AND INDUCE ONCOLOGIC (K562) CELLS APOPTOSIS IN VITRO

Huang Q, Zhang Y, Zhou DD

Zhongnan Hospital of Wuhan University, Wuhan, China

Objective: To determine the antitumor activity of cord blood CIK (cytokine-induce killer cell) cells and explore whether cord blood CIK cells could induce K562 cells apoptosis in vitro and the effect of G-CSF on cord blood CIK cells.

Methods: We can obtain cytotoxic effector cells from umbilical cord blood mononuclear cells by the timed addition of interferon-γ, interleukin-2 (IL-2) and the monoclonal antibody CD3. These cells termed cytokine-induced killer (CIK) cells. MTT was used to determine the antitumor activity of cord blood CIK cells. Apoptosis was detected by TDT end labelling technique and DNA agrose gel electrophoresis.

Results: The antitumor activity of CIK cells is superior to CD3AK cells. The ratio of K562 cells’ apoptosis induced by CIK cells is larger than that of induced by CD3AK cells. Low dosage G-CSF can partially inhibit the effect of CIK cells, but can elevate the proliferation activity of CIK cells.

Conclusion: Cord blood CIK cells possess potency against K562 cells in vitro.The cord blood induced by proper combination of cytokines not only can enhance the cytotoxicity but also retain the function of reconstitute the hematopoiesis.

1080

Objective: To determine the antitumor activity of cord blood CIK (cytokine-induce killer cell) cells and explore whether cord blood CIK cells could induce K562 cells apoptosis in vitro and the effect of G-CSF on cord blood CIK cells.

Results: The antitumor activity of CIK cells is superior to CD3AK cells. The ratio of K562 cells’ apoptosis induced by CIK cells is larger than that of induced by CD3AK cells. Low dosage G-CSF can partially inhibit the effect of CIK cells, but can elevate the proliferation activity of CIK cells.