STUDY OF THE EXPRESSION OF NF-kB IN
PERIPHERAL BLOOD MONONUCLEAR CELLS OF CHILDREN WITH STERIOD RESPONSIVE
SIMPLE NEPHROTIC SYNDROME
Li C-S, Wang Z, Zhu X-S, Tao Y-H
Department of pediatrics,the Second Hospital, West China Medical
Center, Sichuan University, Chengdu, China
Objective: To
study the expression of nuclear factor-kappa B (NF-kB)
in peripheral blood mononuclear cells (PBMCs) of children with steroid
responsive simple nephrotic syndrome (SRSNS), and its relationship with the
level of serum interleukin (IL)-8. To investigate the relationship between
the expression of NF-kB
and the urinary protein of SRSNS, and the role of NF-kB in the triggering of SRSNS by
respirafory tract viruses.
Methods: The
expression of NF-kB was
detected by electrophoretic mobility shift assay (EMSA) and inhibitory
kappa B alpha (IkBa) by western blot analysis, and the
level of IL-8 was measured by emzyme-linked immunosorbent assay (ELISA).
Results: Compared
with normal children, children with SRSNS in remission stage and children
with other types of glomerular disease, the expression of NF-kB in PBMCs of children with SRSNS in
active stage was significant statistically elevated (P<0.05). There was
a positive linear correlation between the expression of NF-kB and the level of serum IL-8, and the
urinary protein (P<0.05). Otherwise, a negative linear correlation
existed between the expression of NF-kB
and cytoplasma IkBa. The expression of NF-kB in PBMCs of children in remission
stage of SRSNS had no difference with that of other types of glomerular
disease and the controls (P>0.05). The relationship among the expression
of NF-kB in
PBMCs, the serum IL-8 and the urinary protein was not existed in children
of SRSNS in remission stage (P>0.05).
Conclusion: The
elevated expression of NF-kB
in PBMCs of children in active stage of SRSNS has close relationship with
the level of the serum IL-8 and the onset of heavy proteinuria. Our study
demonstrated that the respiratory tract viruses may be the important
trigger of SRSNS. We speculate that the viral transactivation of
transcription through activating NF-kB
may be one of the most important ways of the triggering of SRSNS by
respiratory tract viruses.