STUDY FOR EFFECT
OF MEMANTINE ON HSP70 SYNTHESIS FOLLOWING HYPOXIA-ISCHEMIA
Liu
ZHW, Chen HJ, Jiang MH
Xinhua
Children’s Hospital, Shanghai, China
Objective: To
explore the expression of cerebral HSP70 induced by hypoxia-ischemia(HI) in
neonatal rat brain and effect of Memantine on HSP70 exprssion.
Method: HSP70
positive cells was analyzed by immuonohistochemical method at different
time point after the termination of hypoxia in the four groups of the
neonatal rat with HI, pretreatment with memantine prior to HI, treatment
with memantine immediately following HI, and normal control respectively.
Result: Cells immunoreactive for the HSP70 could be found as
early as 2 h after HI, reached to a peak during 48 to 72h, and declined at
7 days after HI, respectively. HSP70 positive cells in cerebral cortex and
hippocampus in both groups treated with memantine were decreased
significantly in particular at 48h and 72h after HI comparing to that in HI
group
Conclusion: HI
was a very sensitive inducer for the synthesis of cerebral HSP70 that exhibited
the course of growth and decline. There was a peak of HSP70 synthesis
during 48~72h and reduced markedly at 7 days after HI. It was noticed after
memantine use that the synthetic amount of HSP70 decreased obviously at
each time point in particular at 48h and 72 h following HI.