STUDY FOR EFFECT OF MEMANTINE ON HSP70 SYNTHESIS FOLLOWING HYPOXIA-ISCHEMIA

Liu ZHW, Chen HJ, Jiang MH

Xinhua Childrení»s Hospital, Shanghai, China

 

Objective: To explore the expression of cerebral HSP70 induced by hypoxia-ischemia(HI) in neonatal rat brain and effect of Memantine on HSP70 exprssion.

Method: HSP70 positive cells was analyzed by immuonohistochemical method at different time point after the termination of hypoxia in the four groups of the neonatal rat with HI, pretreatment with memantine prior to HI, treatment with memantine immediately following HI, and normal control respectively.

Result: Cells immunoreactive for the HSP70 could be found as early as 2 h after HI, reached to a peak during 48 to 72h, and declined at 7 days after HI, respectively. HSP70 positive cells in cerebral cortex and hippocampus in both groups treated with memantine were decreased significantly in particular at 48h and 72h after HI comparing to that in HI group

Conclusion: HI was a very sensitive inducer for the synthesis of cerebral HSP70 that exhibited the course of growth and decline. There was a peak of HSP70 synthesis during 48~72h and reduced markedly at 7 days after HI. It was noticed after memantine use that the synthetic amount of HSP70 decreased obviously at each time point in particular at 48h and 72 h following HI.

 
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