PROTECTIVE EFFECTS
OF ESTRADIOL ON IMMATURE MYOCARDIUM
Tan JN1, Huang XY2, Jiang ZY2, Han L1
1 Anzhen
Hospital, Beijing, China
2 Hubei
Medical University, Wuhan, China
Objective: To
evaluate the protective effects of Estradiol on immature myocardium of
rabbits from ischemia/reperfusion injury.
Methods: The
isolated perfused heart model in a Langendorff preparation was used in
thirty-two 3-4 weeks-old rabbits which were randomly divided into four
groups, C group (Control group, only St ThomasII solution was used in
cardiac arrest), E group (10－6M
17b-estradiol
was contained in St ThomasII solution), B group (the blocker of estradiol,
10－6M 4-H-Tamoxifen was
contained in St ThomasII solution) and EB group (both 10－6M E2 and
10－6M 4-H-tamoxifen was
contained in St ThomasII solution). After 60 minutes cardiac arrest and 90
minutes reperfusion, the heart funtion (CF[coronary flow], LVDP [left
ventricular developed press], ±dp/dtmax
), water content, ATP and MDA content of myocardium, creation
phophate kinase(CPK) leakage of coronary effluent and histologic studiesof
four groups were compared.
Results: The percent recovery
of cardiac function in E group is better than other three groups
(p<0.05). The myocardial water content, MDA content and CPK leakage of
coronary effluent were lower in E group (p<0.05).The myocardial ATP
content was higher than that of the other three groups (p<0.05).
Conclusion: Estradiol
produces good protective effects on immature rabbit myocardium from
ischemia/reperfusion injury.