IGF-1 ON THE EXPRESSION OF GENE ASSOCIATED WITH
HYPOXIC ISCHEMIC BRAIN INJURY IN THE NEONATAL RATS
Lee
H-S, Byen S-H, Chung Y-H
Chungnam
University Hospital, Taejon, Korea
Objective: This study was
designed to investigate the effect of IGF-1 after hypoxic ischemic brain
injury on neuronal cell necrosis, apoptosis and expression of proapoptotic
and antiapoptotic proteins.
Methods:.To make rats hypoxic
ischemic insult, rats were exposed to 8% oxygen at 37C for 2hours with
ligation of carotid artery. Treated
group received intraperitoneal injection of IGF-1 20ug 2 hours after
hypoxic ischemic injury and control group not received, were examined for
necrosis, apoptosis and Bax and Bcl-2 protein expression at 24 hours and 72
hours in both groups.
Results: 1. Necrosis was less in IGF-1 treated group than
control group and was more prominent in CA1 neurons than CA3 neurons. 2.
The apoptosis was significantly less in IGF-1 treated group 3. Bax protein
expression was prominent in control group, especially at 72 hours. The
IGF-1 treated group showed lesss Bax potein expression than control group
and the Bax protein expression was prominent especially at 72 hours after
the hypoxic brain injury in the control group.4. Bcl-2 protein expression
was not detected in both group.
Conclusions: The results from this study suggests that
exogenous systemic IGF-1 had neuroprotective effect by inhibition of
up-regulation of Bax protein expression after hypoxic ischemic brain
injury.