ESTABLISHMENT
AND CHARACTERIZATION OF HUMAN ACUTE MYELOID LEUKEMIA SCID MOUSE MODEL
An SH, Jin XQ
Institute of
Pediatrics, Children’s Hospital, Chongqing University of Medical Sciences,
China
Objective: To explore the effective method of
establishing human myeloid leukemia mouse model the severe combined
immunodeficient (Scid) mice were transplanted by human myeloid leukemia
cell line (HL-60).
Methods: HL-60 cells obtained by
conventional subculture were injected into the Scid mice by intravein (i.v)
or intraperitoneum (i.p). The
Scid mice in group A were inoculated i.p with 5×105 –5×106HL-60 cells; The Scid mice in
group B, inoculated i.v with 5×105-
1×106 HL-60
cells.
Results: Three weeks after
inoculation there is no significant difference in peripheral blood cell
count between two groups. But the detected rate of the peripheral blood
painted slice with human myeloid leukemia cells (86%) in group B was higher
than that of group A (55%) p<0.01. The positive rates of promyelocytes
in group A and group B were 3.8% and 5.4 respectively. Whereas the
histological assay confirmed that all transplanted-mice of the two groups
developed myeloid leukemia after 30 days, and the extension of organs
infiltration (liver, spleen, marrow etc) of group A was broader than that
of group B. p<0.05. Flow cytometric analysis suggested that the
percentage of cells at G2-M and S stage in both liver and spleen
increased greatly. The HL-60 surface antigen (CD33) positive cells
percentage was 4.15%±0.32and
12.22%±0.53 in the bone marrow of
Scid mice in the group B and group A respectively.
Conclusion: The establishment of
human myeloid leukemia Scid mouse model is available by inoculated i.p with
(5×105) or i.v
with (5×105)HL-60
cells. This model is a useful tool for studying pathogenesis and
experimental treatment of human leukemia.