INFLUENCE OF VV-ECMO ON TOTAL COMPLIANCE AND
SURFACTANT IN HEALTHY SHEEP
Zhang Q, Song GW, Gan XZ, Huang WX, Sun LP, LI H, Ma GS, Zhang XL
Capital Institute of Pediatrics, Beijing,
China
Objective: To explore the effect of
venovenous extracorporeal membrane oxygenation（VV-ECMO）on
lung function and surfactant in healthy sheep.
Methods: Twenty sheep were randomly
divided into ECMO group and blood-supplied group. Standard procedures for
venovenous ECMO were used in experimental animals. Pre-ECMO mechanical
ventilation and ECMO (30ml/kg/min) with required mechanical ventilation
were performed. Total dynamic compliances were assessed at pre-ECMO and 5',
15', 30', 60', 90', 120', 180' during VV-ECMO. After exsanguinated through
internal jugular venous, lungs of both groups were lavaged 3 times with
20ml/kg saline through trachealintubations. Total phospholipid,
phosphatidylcholine, and total protein in lung lavage fluid were measured.
Results: There was no significant
difference between the weight of the two groups. The total dynamic
compliance of pre-ECMO was higher than that of each interval time during
VV-ECMO (p<0.01). There was no significant difference among the total
compliance of each interval time during VV-ECMO. No significant difference
was found between the concentration
of total phospholipid, phosphatidylcholine and total protein in lung lavage
fluid in two groups (P>0.05).
Conclusions:
Total dynamic compliance during VV-ECMO is lower than pre-ECMO. But no
influence is shown on amount of surfactant in sheep during VV-ECMO
procedure in three hours.