THE STUDY
OF MULTI-ORGANS DAMAGE ON MURINE ANIMAL MODEL AFTER INTECTED BY HUMAN
ROTAVIRUS
Yao YY, Ou QQ , Li N
Pediatrics’ Department,
Nanfang Hospital,
The First Military
Medical University, Guangzhou 510515, China
Objective: To study the extraintestinal
infection on the murine animal model.
Methods: Collect the stool sample from the
rotavirus infected patients, through a series cleaning and abstracts the
human rotavirus. Then inoculated the immunodiefiency mice with the
rotavirus, killed the mice in batches to exam the pathologic changes, and
detected the rotavirus RNA by insitu polymerase chain reaction.
Results: There are some
pathology changes in the heart, liver, kidney, and gallbladder of the
rotavirus infected mice, and the rotavirus double-strand RNA was found in
the cell plasma of the liver, kidney, gallbladder and pancreas but was not
found in the lung and brain.
Conclusions: The rotavirus
extraintestinal infection animal model was made successfully. The rotavirus
can invade the cells of liver, kidney, gallbladder and panceas directly.