LOCALIZATION OF MBP1/a-ENOLASE GENE TO
CHROMOSOME 1p36.2-3 AND ALTERATIONS OF THIS GENE IN CHILDHOOD NEUROBLASTOMA
Kong X-T,
Wilkinson DA, Valentine MB, Ragsdale ST, Valentine VA, Rowe ST, and Look AT
Department
of Experimental Oncology, St Jude Children's Research Hospital, Memphis,
TN, USA
Objective:
To
determine whether inactivation of MBP-1/a-enolase, a gene encoding
C-myc promoter binding protein,
contribute to the genesis or progression of childhood neuroblastoma with MYCN amplification.
Methods: Fluorescence in
situ hybridization (FISH) analysis
Results:
Eighteen
neuroblastoma cell lines were examined by FISH analysis. We found that the MBP-1/a-enolase gene was assigned to chromosome bands 1p36.2-3, which
is included in the large regions deleted very frequently in neuroblastoma
with MYCN amplifications.
Seventeen of the 18 lines showed deletion or translocation of one allele of
the MBP-1/a-enolase gene on
chromosome 1, suggesting that the MBP-1/a-enolase gene locus was
consistently affected in neuroblastoma cells, probably contributing to the
inactivation of this gene in neuroblastomas. However, sequence analysis of
the cell lines only remaining one allele of the gene, did not show any
inactivating mutations, suggesting that the MBP-1/a-enolase gene may be not homozygously inactivated in this tumor.
Other mechanisms, together with the deletion or translocation of the one
allele of the MBP-1/a-enolase gene may
contribute to the genesis or progression of neuroblastoma.
Conclusion: Consistent affecting of MBP-1/a-enolase gene in most neuroblastoma cell lines with MYCN amplification found in this
study suggest that MBP-1/a-enolase gene
plays an important role in the pathogenesis of neuroblastomas with MYCN amplification.
|
|
1510