THE STUDY ON
TRANSMISSION OF GROUP B STREPTOCOCCUS INFECTION IN NEONATES
Shen A.D., Si Y.,
Yang Y. H.
Department of
Microbiology, Beijing Children’s Hospital, Beijing, China
Objective To study the transmission of group B streptococcus
(GBS) in the neonatal infections and the value of randomly amplified
polymorphic DNA analysis (RAPD) in the GBS epidemiology.
Methods: Serotyping, DNA-DNA hybridization, E-test, and RAPD
typing methods were used for this investigation. Nine strains were
mother-baby pairs, the three from mothers, six from their neonates with
early and late-onset infection, eighty-eight strains from the vaginas of
pregnant women.
Results: In
the three case, the strains of each mother-baby pair produced identical
serotype (III/R), antimicrobil susceptibility profiles, restriction
fragment length of virulent factors genes ( including alpha and
beta genes negative, scaA 2kb, glnA 6.5kb, scpB2.6kb); In the
two case of early-onset infection, the strains of mother-baby pair have the
identical RAPD pattern ; In the late-onset infection, the strains of
mother-baby pair produced different RAPD pattern, GBS strain isolated form
mother was the RAPD27, GBS strains from blood and CSF of baby were RAPD1.
Conclusion:
These data exhibits the mother-baby vertical transmission in early-onset
and cross transmission in late-onset; RAPD analysis provides a valuable
molecular tool for studying GBS epidemiology.