Text Box: THE EFFECT OF ROTAVIRUS INFECTION ON THE LIVER AND BILE OF BALB/C MOUSE Feng Jiexiong, Li Minju, Tang Hongfeng, et al. Department of Pediatric Surgery, Children,s Hospital, Medical College of Zhejiang University, Hangzhou, China Objective: To evaluate the effect of rotavirus infection on the liver and bile of BALB/C mouse. Method: The SA11 stain of rotavirus was passaged two times, and TCID50 was up to 10-5. Twenty-four adults BALB/C were divided four groups, one group as control, the other three group were inoculated with 10-1,10-2, or 10-3 TCID50 rotavirus through intraperitoneal route. The same dose of rotavirus was inoculated to the pregnant mice. Newborn of mice, except one group for the control, three groups were infected rotavirus as adult groups, and one group inoculated 10-2TCID50 rotavirus for five days. The weight of each mouse was evaluated, and the color of skin, stool and urine was also inspected to estimated the degree of jaundice. The samples included liver and bile of every group were collected after 4 days interval and fixed in formalin, embedded in paraffin, and stained with HE. Results: All mice were survival, and no difference of weight was seen in every matched group. No evidence of jaundice was obtained during experiment. The degree of pathologic change of liver and bile in every experimental group was similar. The degeneration and swelling of liver cell could be found after 4 days of inoculation, and the evident infiltration with neutrophils was seen on the 7days after inoculation. Then, the liver cell recovered spontaneously, and the infiltration with lymphocyte was found on the days of 21 days. One the 28 day after infection, the morphological manifestation of liver recovered as the same as the control group had. The pathology of bile was similar to the liver cells, and no stricture or atresia was seen during experiment. Conclusion: The acute inflammation of liver and bile can be induced by the way of inoculation with SA11 strain rotavirus in BALB/C mouse. But this pathology can be recovered spontaneously, which is very similar to the pathology of hepatitis of newborn in human being. 1702