IN VITRO STUDY OF ANTI-LEUKEMIA EFFECTS OF IL-4 GENE
MODIFICATION
XD Zhao, XQ Yang
& CR Li.
Children’s Hospital,
Chongqing University of Medical Sciences,
Chongqing 400014, China
Objective: To observe effects of
IL-4 gene modification on the growth of leukemic cell lines, HL-60 and
K562, and on the functions of T lymphocyte induced by gene modified
leukemic cell lines.
Methods: IL-4 gene was introduced
into above cell lines via the retrovirus-mediated gene transfer method.
Compared with the wild type, vector-tranfected HL-60 and K562 cells, the
proliferation, cell death of IL-4 gene-modified leukemic cells (H-IL-41
and K-IL-41) and the proliferation of normal PBMC induced by
them were detected. RT-PCR and 51Cr release test were applied to
detect IL-2, IL6 and IFN-γmRNA
expression in PBMC and to measure the killing activity of specific CTL,
respectively.
Results: The maximum levels of IL-4
production in IL-4 gene modified HL-60 and K562 cells, H-IL-4+
and K-IL-4+, reached 65.4pg/106cell/24h and
106.6pg/106cell/24h, respectively. The proliferative of H-IL-4+
and K-IL-4+ cells was inhibited significantly and cell death of
K-IL-4+ cell increased. The proliferation capacity of cytokines
production in PBMC induced by leukemic cells and the killing activity of
specific CTL also increased dramatically.
Conclusion: IL-4 gene modification
not only inhibited the growth of leukemic cell lines directly, but also
controlled the growth by promoting lymphocyte function and the killing
activity of specific CTL.