EXPLORATION TO THE APPROACH OF DETECTING FETAL
GENETIC INFORMATION IN MATERNAL PERIPHERAL BLOOD CELL AND PLASMA
Zhu J, Kang GF, Wang NS, Deng B
Children¡¯s Hospital,
Chongqing University of Medical Sciences, Chongqing, China
Objective: This study was to
explore the feasibility of detecting fetal genetic information in maternal
peripheral blood cell and plasma.
Methods: We performed nest-PCR to amplify the special fragment of
SRY gene and identified it by DNA sequencing in 50 peripheral blood cells
and plasma samples from pregnant women.
Results: Fetus-derived Y chromosome
specific fragment were detected in 21(75%) of 28 maternal peripheral blood
cells and in 9(32%) of the maternal plasma samples from women having male
fetuses. No false positive result was observed among those pregnant women
with female fetus.
Conclusion: Detecting fetal DNA in
peripheral blood cell and plasma from pregnant women could be a feasible
approach of non-invasive prenatal diagnosis. Further clinical trials for identifying
the fetus with Mediterranean anemia by this method are underway.