STUDY ON THE RELATIONSHIP BETWEEN HUMAN CYTOMEGALOVIRUS INFECTION
AND HIRSCHSPRUNG’S DISEASE
Chen LeiLing,
Jin XianQing
Department of General
surgery, Children's Hospital, Chongqing University of Medical Sciences, Chongqing,
China
Objective: The
cause of this aganglionosis remains uncertain. At present human
cytomegalovirus (HCMV ) is held to be the most important one among viruses
known by now which contribute the congenital malformation and mental
dysplasia in infants and young children. Because of no information on the
distribution of HCMV in the colon from HD till now, HCMV infection could
not be sure to be the cause of HD. The objective in this issue is to
understand the distribution of HCMV DNA in the colon tissues from HD.
Method: 21
colon tissue samples from HD after operation embedded in paraffin were
examined to know the distribution of HCMV DNA using in-situ polymerase
chain reaction (ISPCR).
Results: The
distribution of HCMV was very abroad in tissue from the colon of HD, but
the major site was the nuclei and cytoplasm of fibroblast. The positive
ratio HCMV DNA in aganglionic bowel was 30.8% in HD by ISPCR.
Conclusion: HMCV
infection may be related the cause of HD, but the further study is needed
to see how HCMV hamper the development of the enteric ganglia.