RESEACH ON THE LONG-TERM EXPRESSION OF
MDR1GENE WITH A MURINE BONE MARROW TRANSPLANTATION MODEL
Kang Q, Jin XQ, Li YC, Xu JL
Institute of Pediatrics, Children¡¯s
Hospital, Chongqing University of Medical Sciences, China
Objective: To investigate the long-term
expression of MDR1 gene in hematopoietic cells with a murine bone marrow
transplantation model and to explore if it is a stable and effective
approach that MDR1 gene was transferred into hematopoietic cells to provent
myelosuppression in chemotherapy.
Methods: MDR1 gene was transferred into
hematopoietic cells of murine bone marrow with plasmid pHaMDR1/A by the
mediation of retrovirus vecter.
The expression and function of MDR1 gene in different phase was
tested in vivo by bone marrow transplantation murine model.
Results: MDR1 gene was transferred into
hematopoietic cells of murine bone marrow successfully, within 5 months,
stable and effective expression could be tested in hematopoietic cells of
murine bone marrow in vivo. the ratio of MDR1 gene transferred cells in
recipient's hematopoietic cells decreased in every month: 8%, 8%, 7.5%, 4%,
3% .
Conclusion: it is a stable and long-term approach
that MDR1 gene is transferred into hematopoietic cells to prevent
myelosuppression in chemotherapy.