AN EXPERIMENTAL STUDY ON EFFECT OF BFGF ON CHANGES OF HISTOLOGY OF SPLIT THICKNESS SKIN

Fu Yuexian, Xiang Daili, Mi Can, etal

Department of plastic SurgeryChildren’s Hospital of Chongqing Medical University, Chongqing, China

 

Objective: To investigate the effect of the extrinsic bFGF on changes of histology of experimental split thickness skin.

Methods: A model of the autografting of split thickness skin on guinea pig was used. All the guinea pig were randomly divided into four groupsi.e. A. normal control group. B. 300Au bFGF/ml group. C. 600 Au bFGF/ml group. D. 900 Au bFGF/ml group. At the times of 1, 2, 4 postoperation weekthe dermal grafts were excised. Then the dermal grafts were observed with the methods of routine histology and electron-microscope. The amounts of capillary and the density of fibroblast of the derma were computed.

Results: In 1 postoperation weekthe multiplication of the capillaryfibroblast and collagenous fiber were appeared in all groups and those were obvious in D groupexcept for A group. In 2 postoperation weekthe obvious multiplication of the capillaryfibroblast and collagenous fiber were appeared in B, C, D groupsthose were especially obvious in D group. In 4 postoperation weekthe light degree of multiplication of the capillary, fibroblast and collagenousfiber were appearedthe defference among each group was not obvious.

Conclusion: Extrinsic bFGF has the effect of promoting the multiplication of the capillaryfibroblast and collagenous fiber of experimental split thickness skinthe effect would be helpful to the healing of the skin and the repair of the wound.

 

 

 
1772