DETECTION
OF CHLAMYDIA TRACHOMATIS BY LIGASE CHAIN REACTION
Wei H,
Wu S
Children¡¯s
Hospital, Chongqing Medical College, Chongqing, China
Objective: To
develop a nucleic amplication method for the detection of Chlamydia
trachomatis DNA by ligase chain reaction (LCR).
Methods: Two
pairs of probes were designed according to the gene encoding major outer
membrane protein (MOMP). DNA fragments of different species and other
bacterial were detected with LCR and routine polymerase chain reaction
(PCR).
Results: Using LCR, DNA fragments of 54bp
were amplified from five different species. The sensitivity could be
improved to 2 elementary bodies. LCR detected ten¨Cfold EB than PCR. No
signal was observed when C.psittaci, C.pneumoniae and other bacterials were
used as templates.
Conclusions: The
results showed that LCR is sensitive and specific for detection of
C.trachomatis.