EFFECT OF TPO/IL-11 GENE MODIFIED STROMAL CELLS ON
EXPANSION OF CD34+CD38- HEMATOPOIETIC PRIMITIVE PROGENITOR CELLS
Yang G1,
Li Liang2, Pei XueTao2, Zhou YaDe1, Ye
ChuanZhong2, Feng Kai2
1 Department
of Hematology/Oncology, Chongqing Children's Hospital, Chongqing University
of Medical Sciences, China
2 Department
of Experimental Hematology, Institute of Radiation Medicine, Beijing, China
Objective: To
study the effects of TPO and/or IL-11 gene modified stromal cells on
expansion of CD34+ hematopoietic stem/progenitor cells from cord
blood.
Methods: Retroviral
vectors carrying TPO or IL-11 gene were constructed and used to transfect
the stromal cell line HFCL. The TPO and/or IL-11 mRNA was assayed by
Northern blot after transduction. By constrast with expansions supported by
non-modified stromal cells, CD34+ hematopoietic stem/progenitor
cells from blood cord were expanded on gene-modified stromal cells for 7
days. The phenotype of CD34+CD38- primitive
progenitors was detected by flow cytometry.
Results: The
stromal cell line HFCL could expressed the TPO and/or IL-11 gene after
transfected by the retrovirus. The percentages of CD34+CD38-
primitive progenitors in the presence of TPO gene modified HFCL(1.8±0.24)%, in the presence of
IL-11 gene modified HFCL(1.62±0.23)%,
and in the two gene cotransduced HFCL(2.45±0.28)% were higher than in the nontransduced
HFCL(0.8±0.23)%.
Conclusion:
The stromal cells modified by TPO and/or IL-11 gene had ability to enhance
ex vivo expansion of CD34+ hematopoietic stem/progenitor cells
from cord blood.