STUDY ON VERTICAL TRANSMISSION OF CHLAMYDIA
TRACHOMATIS USING PCR AND DNA SEQUENCING
Wu Shixiao, Shen Li, Liu Guanxin
Children¡¯s Hospital, Chongqing, China
Objective:
To investigate the vertical transmission rate of Chlamydia Trachomatis (CT)
in Chongqing, China.
Methods:
Specimens taken from 278 women and from their 79 infants were Examined
by cell culture, polrmerase chain rection(PCR) and DNA sequence Analysis.
CT was isolated in McCoy cell culture. CT DNA was extracted With a modified
NaI method after cloning, recombinant plasmids were used For sequence
analysis with the dideoxy chain termination method.
Results:
10.8%(30/278) of the cervical cultures of pregnant women were Positive to
CT,while the positive rate tested by PCR was 14.0%( 39/278). The vertical
transmission rate of CT was 55.0%(11/20). The incidence of Conjunctivitis
and pneumonia in infants were 27.3% and 18.2%,respectively. DNA sequences
of CT isolated from the cervix of a mother and the nasopha-Rynx of her baby
were identical.
Conclusion:
CT infection is quite common in Chongqing,China. Our report Is the
first report of CT vertical transmission proced by DNA sequences Analysis.