IMMUNOMODULATORY EFFECTS
OF INSULIN-LIKE GROWTH FACTOR-1 (IGF-I) ON CORD BLOOD IMMUNOCOMPETENT CELLS
Wang YH, Li CR, Yang
XQ
Department of
Immunology, Chongqing child hospital, Chongqing, China
Objective: To explore the
modulatory effects of IGF-I on cord blood immunocompetent cells.
Methods: 3H-TdR
incorporation and 3H-TdR releasing methods were used to observe
the effect of IGF-I on proliferation of T cells, killing activity of natual
killer (NK) cells and cytotoxic T lymphocytes(CTLs) in cord blood
mononuclear cells (CBMC).
Results:
1.
IGF-I significantly enhanced mitogen stimulated DNA synthesis and promote T
cell proliferation in CBMC.
2.
IGF-I significantly enhanced killing activity of basal or IL-2 activated NK
cells in CBMC.
3.
IGF-I significantly increased killing activity of CTL in CBMC.
Conclusion: IGF-I could play a
positive modulatory role in immunocompetent cells in CBMC and it¡¯s
promising to be used as immunostimulatory reagent in clinic. Because of the
complexity of the network of human neuroendocrine and immune systems,
further study should be required to understand the effects of IGF-I on main
organs and systems of body in addition to immune system.