文本框: NON ENZIMATIC ANTIOXIDANT CAPACITY IN CHILDREN 
AFFECTED OF TYPE 1 DIABETES MELLITUS (IDDM)
Campoy C, Baena RMª, Blanca E, Fernandez JM, Molina-Font JA, Bayes R
Department of Paediatrics, School of Medicine, University of Granada, Spain

Adequate plasma and tissue levels and correct intake of vitamins A and E and selenium are needed to obtain optimal results in their cardiovascular protective role. The aim of this study is to analyse the non-enzymatic antioxidant capacity by measuring vitamins A and E, and selenium nutritional status in IDDM children. 
METHODS: Subjects: Group I (G-I) formed by 47 randomised IDDM children, matched for age and sex with 16 healthy children [group II (G-II)]. Biochemistry: Plasma Vitamins A (Vit Ap) and E (Vit Ep) in µg/dl (HPLC); Vitamin E in erythrocytes (Vit Ee) in µg/ml of packing erythrocytes (HPLC); Retinol binding protein (RBP) in mg/dl (nephelometry); % glycosilate haemoglobin (HbAlc) (HPLC). Spectropho-tometry: Serum total cholesterol (TC), triglycerides (TG) (mg/dl) and selenium (µg/L). Statistical analysis: ANOVA and Student´s “t” test for unpaired data were done. RESULTS (G-I vs G-II): RBP:2.39±0.13 vs 2.07±0.08*; Vit Ap:1.03±0.03 vs 1.17±0.06*; Vit Ap/TC:0.60±0.02 vs 0.71±0.04*; Vit Ap/TG:2.09±0.13 vs 2.08±0.19; Vit Ep: 10.02±0.32 vs 8.72±0.36*; Vit Ee: 2.89±0.08 vs 2.63±0.09*; Vit Ep/Vit Ap: 10.00±0.39 vs 7.67±0.45*; Vit Ep/TC: 5.73±0.13 vs 5.24±0.20; Vit Ep/total lipids: 4.33±0.09 vs 3.84±0.16*; selenio: 73.59±3.03 vs 65.32±2.82; *=p<0.05. CONCLUSIONS: The increase of RBP in IDDM could be explained by a protective mechanism of this carrier in order to improve the offer of the lower vitamin A plasma concentrations to the tissues. The ratio Vit Ap/TC was lower in IDDM children than in healthy one; this suggests a relative vitamin A insufficiency. Our data shows that increased plasma and erythrocytes Vit E in IDDM may be due to the higher oxidative stress, and probably an increased turnover and/or the increment of absorption from the diet. We conclude, that while vitamin A is rapidly consumed in IDDM children, Vit E maintains its antioxidant capacity in the early periods of the illness.
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