文本框: PLASMA ACYLATION STIMULATION PROTEIN IN OBESE CHINESE CHILDREN
Wang HW1, Cheng PX1, Hu XF1, Cianflone K2
1Dept. of Pediatrics, Tongji Hospital, Huazhong University of Science and 
Technology (HUST). Wuhan, China
2Dept. of Cardiovasology, Royal Victoria Hospital, Mcgill University. Montreal, Canada

Objective: Acylation stimulation protein (ASP) is an adipocyto-derived protein that has potent anabolic effects on human adipose tissue for both glucose and free fatty acid storage. Plasma levels of ASP have been determined only in normal and obese caucacian individuals. In this study, we investigated the plasma concentrations of ASP in obese Chinese children. We also observed the relationship between ASP and other three parameters, plasma triglyceride (TG), total cholesterol (chol) and leptin. The goal is to find a new direction for children obese prevention and treatment.
Methods: ASP was measured in fasting samples obtained from 50 age-matched controls and 100 obese children by ELISA method. At the same time, the plasma TG, Chol were measured by commercial enzymatic colorimetric assay, as well as the leptin was observed by radioimmunoassay methods.
Results: In the obese children subject, plasma ASP was significantly higher than the controls' (68.27±2.13 nmol/L obese children versus 24.36±2.66 nmol/L control, P<0.001). The level of ASP was significantly higher in 7~14 years old obese girl group compared to the obese boy with the same age (76.65±3.96nmol/L versus 58.97±4.64nmol/L, P<0.05.) Linear regression analysis showed an association between plasma ASP and plasma TG (P<0.05), as well as Chol (P<0.05) and leptin (P<0.05).
Conclusion: The data first expound the plasma ASP levels in obese Chinese children. It demonstrates a relation between the ASP pathway and other metabolic determinants or obesegenetic hormone. ASP may play an important role in children's obesegenesis.
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