THE STATES OF　FUNCTION OF T CELLS　AND ROLES OF　CYCLOSPRINE A IN CHILDREN WITH

HENOCH-SCHONLEIN PURPURA

LI Q, Yang X-Q, LI Y-B, Wang L-J

Children’s Hospital, Chongqing University of Medical Science，Chongqing，Chain

Objective: To observe the states of　function of T cells and to investigate the roles of Cyclosporin A(CsA) in T cells in the children with Henoch-Schonlein purpura(HSP) in the　acute phase.

Methods: The cells count of CD30 expression in Peripheral blood mononuclear cell(PBMC) were determined by flow cytometry. IgA, IgG, IgM, IgE，Ig subsets and cytokines were detected by radial immunodiffusion and ELISA.

Results: The serum IgA and IgE levels, and IgG subsets, IgE, IL-4 and IL-5 levels in the supernatants of cultured PBMC of HSP patients were increased significantly in contrast to the normal controls. IL-2 and IFN-γwere decreased. The raised of IL-5 and IL-4 in the supernatants of cultured PBMC of HSP patients were related to the increased of IgA（γ=0.63）and IgE（γ=0.87） in serum. The leveals of high TH2 cytokines such as IL-4,IL-5 and IL-6could be affected by CsA in vitro. The TH1 cytokines further were inhibited.

Conclusions: the dysfunction of T cell were related to the pathogenesis of HSP patients. The inhibition of CsA on T cells function of HSP patients were showed. To inhibited TH1 function mainly.

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