The significance of expression of Fas
antigen and Bcl-2 protein in the kidney of adriamycin induced nephrosis in
rats
Sun Liangzhong, Yue ZH, Xu HS, Chen SM,
Liang M, Tang JR
Department of Pediatrics, The First Affiliated
Hospital,
Sun Yat-Sen University of Medical Sciences,
Guangzhou, China
Subject:
To further understanding the mechanism of apoptosis in the process of
glomerulosclerosis.
Methods: 72 male SD rats aged 8~10
weeks were randomly divided into three groups (A, B, C), each group had 24
rats. Rats in group A and B were given adriamycin 2mg/kg intravenously on the
1st and the 21st day respectively. At the same time, an equal volume of
natural solution was given to the rats in the group C by the same method. After
the second injection, rats in group A received enalapril (50mg/L) in their
drinking water. 4 rats in each group were killed every 8 weeks from the 4th
week after the second injection. Apoptotic cells in the kidney samples were
detected by in the TUNEL method and the electronic microscopy. The
expression of Fas antigen, Bcl-2 protein and AT2R in kidney samples were
detected by immunohistochemical staining respectively; Gray values of
positive staining in glomeruli were obtained by computer image analyzer and
quantified as positive unit (PU).
Results: In group A and B, glomerulosclerosis was emerged and
the number of apoptotic cells in glomeruli increased after the 20th
week. But glomerulosclerosis in the group A was not so severer compared
with that in the group B at the 20th week. Glomerular cell
apoptotic index (GAI) in group A at the 20th and 28th
week were less than those in group B. The expression Fas antigen was
significantly increased and the expression of Bcl-2 protein was
significantly decreased in glomeruli in group A and B after the 20th
week. The differences of Fas antigen expression in glomeruli between group
A and B had not significant. But the expression of Bcl-2 protein in
glomeruli was partially reserved in group A compared with group B. We could
not detect any expression of AT2R in glomeruli in three groups. The PUs of
Fas antigen and Bcl-2 protein in glomeruli in group A and B were was significantly
correlated to glomerular sclerotic index (GSI) and GAI repectively.
Conclusion: In the process of
gromerulosclerosis in adriamycin-induced nephrosis, glomerular cell
apoptosis might be induced by the increased expression of Fas antigen and
the decreased expression of Bcl-2 protein, but not the expression of AT2R.
Enalapril interposing could ameliorate glomerulosclrosis, and partially
reserving Bcl-2 expression in glomerular by which might inhibit glomerular
cell apoptosis.