IL-4 AND IL-10 ENHANCE DERMATOPHAGOIDES
FARINAE-SPECIFIC IgE AT THE LEVEL OF THE EBV-IMMORTALIZED B CELL LINE
Noma T,
Yoshizawa I, Saeki T, Sugawara Y, Ishikawa Y, Ogawa N, Kawano Y, Matsuura N
Department of
Pediatrics, Kitasato University School of Medicine, Sagamihara, Japan
Objective: Interleukin (IL)-10 accelerates IgE
production by anti-CD40- and IL-4-stimulated PBMC by enhancing IL-6
production through activation of T lymphocytes or antigen-primed spleen
cells, in addition to its role as a regulator of the inflammatory
responses. We extend the investigations of the mechanisms enhancing IgE
synthesis.
Methods: We determined the effect of IL-10 on the
secretion of Df-specific IgE by the EBV-immortalized cell line, K7 cells.
Results: Df-pulsed autologous T cells, as well as the
supernatants of these cultures, mediated increased the synthesis of
Df-specific IgE production. Antigen-specific IgE was also enhanced when K7
cells were treated with anti-CD40 antibody and with both IL-4 and IL-10, or
with IL-4 and IL-10. The treatment of K7 cells with anti-CD40 antibody and
IL-4, or anti-CD40 antibody and IL-10 did not increase IgE production.
Conclusion: The results indicate that IL-10, a
Th2-type cytokine, directly affects the mature B cells that produce IgE,
and that the secretion of IgE is increased by treatment with IL-10 in cells
that are stimulated with anti-CD40 and IL-4 at the level of the
EBV-immortalized cell line, already switched to the production of IgE.