THE CHANGES OF
STOMACH’S PROTEASES DURING EARLY POSTNATAL PERIOD
Hasanova G, Velieva G
Medical
University, Baku, Azerbaijan
It is found that at
new-borns the acidity of gactric juice is provided not by HCI, but by milk
acid, which doesn’t make optimal pH for activity of stomach;s proteases.
Owing to this, we decided to study how the protein’s digestion happens at
milk age.
Objective: The experiments were made on 38 puppies
at the age of 2-3, 12 and 20 days.
Methods: The proteolytic activity of different secretor
spheres of gastric mucosa was investigated by the method of
dick-electrophoresis on PAAH. For the exposure of proteolytic active
fractions the hels were incubated into 0,1% of haemoglobin solution in 1M
CH3COONa-HCI buffer at pH 1,0, 5,2, 7,0 and 8,5 during 3 hours
with the temperature 370C, washed with water, fixed during 1
hour and painted. Against the dark background of haemoglobin, which was
penetrated into hel, there were seen the light lines of proteases
splintered the haemoglobin. The hels densitometrated. We determined the
proteolytic activity proteases’ isoforms by the way of weigh on torsion
scale the densitogram parts, which are suitable to separate fractions. The
results received by pH 1,0 were taken as control.
Results: As appeared there were additional gastric
proteases activated during the weakening of acid reaction of environment at
early postnatal ontogenesis. At 12 days old the number of proteases reached
10 and at the age of 20 days it decreased. The maximum quantity of isoforms
was found put at pH 5,2.
Conclusion: The investigated additional proteases
isofractions can be considered as the consequence of adaptation to the only
food of this period --mother milk.