EVALUATION OF OXIDATIVE STRESS IN CHILDREN WITH
GASTROESOPHAGEAL REFLUX
K.Wąsowska-Królikowska,
M.Modzelewska-Hołyńska
Clinic of Pediatric
Gastroenterology and Allergology, Medical University of Lodz, Poland
Objective: The aim of study is the evaluation of selected
components of antioxidative barrier in children with gastroesophageal
reflux and esophagitis.
Methods: Research includes 20 children in the age from 2
months to 16 years. Gastroesophageal reflux was diagnosed on the basis of
24-hours pH-metry and endoscopic with histology examinations of upper
gastrointestinal tract. We investigated activity parameters of antioxidant
barrier: superoxide dismutase, glutathione peroxidase, catalase and
conjugated diens in plasma and gastric biopsy homogenates. In our study the
intensity of peroxide lipid oxidation was calculated by concentration of
malonic dialdehyde (MDA) in plasma. The investigations were performed
before and after 2 months treatment.
Results: 11 (55%) with 20 children with gastroesophageal reflux
presented macroscopic variables, which prove esophagitis. In 16 children
inflammatory process was attested in histology study of specimens. After 2
months medical treatment 15 patients with 20 (75%) had not clinical
symptoms or reduce intensification. Our study showed lower activity of
antioxidative enzymes in children with esophagitis opposites children with
gastroesophageal reflux without esophagitis. Higher concentration MDA in
plasma and homogenates had children with esophagitis and increased after
treatment.
Conclusion: Oxygen derived free radicals are probably
involved in pathogenesis of esophagitis in children with gastroesophageal
reflux. Usage of antyreflux and anti-inflammatory therapy caused reduces of
oxidation stress.