INFLUENCE OF HEPATITIS B VACCINE ON THE LEVEL OF
BETA-2-MICROGLOBULIN IN NEWBORNS SERUM
Szponar E1, Furmaga-Jablonska
W1, Chrzastek-Spruch H1, Polkowska G1,
Billewicz-Kraczkowska A1, Jagiello-Wojtowicz E2
1 Department of Paediatrics, Medical University School of
Lublin, Poland
2 Department of Toxicology in Lublin, Poland
Objective: The aim of the study was to evaluate the influence of Hepatitis B
vaccine (Engerix B) on the level of beta-2-microglobulin (b2m) in the new-borns' serum.
Methods: The study included 47 healthy and full-term new-borns with breast
milk jaundice. New-borns were divided into two groups: - 21 new-borns who
were vaccinated and the control group - 26 new-borns were not vaccinated.
The b2m assay was performed with fluoroimmunologic method by using ready
DELFIA sets. Statistic was done with t-Student test. P < 0,05 was
regarded as significant.
Results: The b2m concentration in the blood serum of new-borns who were vaccinated
was: on the third day 3,41 ±0, 22 mg /l, on the sixth day 2,83±0, 19 mg /l,
and on the ninth day 3,35±0, 24 mg /l. In the control group the level of b2m was on the third day 3,19±0,10 mg /l, on
the sixth day 2,75 ± 0,11 mg /l and on the ninth day 2,63±0, 10 mg /l. In
the group of non-vaccinated new-borns significant decrease of b2m level was noticed between the third and
the sixth day (p<0,05) as well as between the third and the ninth day
(p<0,001). Additionally a significantly higher level of b2m was confirmed on the ninth day in the
vaccinated new-borns as compared to the non-vaccinated ones (p<0,001).
Conclusions: Significant increase of
b2m level noticed in the new-borns following
the Engerix B vaccination may be the evidence of contribution of this
protein to immunological processes.