INFLUENCE OF HEPATITIS B VACCINE ON THE LEVEL OF BETA-2-MICROGLOBULIN IN NEWBORNS SERUM

Szponar E1, Furmaga-Jablonska W1, Chrzastek-Spruch H1, Polkowska G1, Billewicz-Kraczkowska A1, Jagiello-Wojtowicz E2

1 Department of Paediatrics, Medical University School of Lublin, Poland

2 Department of Toxicology in Lublin, Poland

 

Objective: The aim of the study was to evaluate the influence of Hepatitis B vaccine (Engerix B) on the level of beta-2-microglobulin (b2m) in the new-borns' serum.

Methods: The study included 47 healthy and full-term new-borns with breast milk jaundice. New-borns were divided into two groups: - 21 new-borns who were vaccinated and the control group - 26 new-borns were not vaccinated. The b2m assay was performed with fluoroimmunologic method by using ready DELFIA sets. Statistic was done with t-Student test. P < 0,05 was regarded as significant.

Results: The b2m concentration in the blood serum of new-borns who were vaccinated was: on the third day 3,41 ±0, 22 mg /l, on the sixth day 2,83±0, 19 mg /l, and on the ninth day 3,35±0, 24 mg /l. In the control group the level of b2m was on the third day 3,19±0,10 mg /l, on the sixth day 2,75 ± 0,11 mg /l and on the ninth day 2,63±0, 10 mg /l. In the group of non-vaccinated new-borns significant decrease of b2m level was noticed between the third and the sixth day (p<0,05) as well as between the third and the ninth day (p<0,001). Additionally a significantly higher level of b2m was confirmed on the ninth day in the vaccinated new-borns as compared to the non-vaccinated ones (p<0,001).

Conclusions:  Significant increase of b2m level noticed in the new-borns following the Engerix B vaccination may be the evidence of contribution of this protein to immunological processes.

 
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