THE PRIMARY IMMUNE RESPONSE PATTERN OF THE TWO GROUP MICE WHICH HAD BEEN IMMUNIZED WITH A SINGLE DOSE 500 MG OR 1000 MG OF E PROTEIN OF DENGUE VIRUS

*Candra Bumi, *Fedik A Rantam, **Soegeng Soegijanto, **Nur Hidayah

*Tropical Disease Center, Surabaya

**Child Health Department of Dr.Soetomo Hospital Surabaya

 

Objective: To express the IgM and the IgG antibody level of primary immune response in animal model after injected E protein of Dengue virus infections.

Method: This study used the strains of BALB/C of 40 mice (mus musculus) that had never been infected with dengue virus. The mice were divided three groups. The first group consisted of 10 mice that were immunized with PBS as control group. The second and the third group consisted of 15 mice respectively. The second group was immunized with E protein fraction 500-mg dosage per each whereas the third group was immunized with E protein fraction 1000-mg dosage per each. The serum of mice were collected for 4,7,10,15,22 and 30 days after immunized and analyzed with ELISA for measuring content of immunoglobulin produced. The fifth day the blood of mice was performed flowcytometri analysis to determine cellular immunity.

Result: There were differences significantly between IgM level and IgG level as well as among the immunoglobulin level of IgG, IgG1, IgG2a and IgG2b both in the 500 mg and the 1000 mg dosage (p < 0.005). There increased 3.7% and 4.9% of CD4 and CD8 respectively of cellular immunity.

Conclusion: The primary immune response in the study showed there were differences significantly between IgM level and IgG level as well as among the subtype of immunoglobulins both in the 500 mg dosage and the 1000 mg dosage. There also increased CD4 and CD8 of cellular immune.

Key word: Primary immune response, E protein of Dengue virus, Immunoglobulin

 
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