STUDY OF THE SIGNALING
TRANSDUCTION OF NEONATAL UMBILICAL CORD BLOOD LYMPHOCYTES INDUCED BY
ANTI-CD3 ANTIBODIES
CHEN TX, HE ZJ, WU SM,
Ying DM
Shanghai Institute
for Pediatric Research, Xinhua Hospital, Shanghai Second Medical
University, Shanghai, China
Objective: To study on the dysfunction mechanism of the signaling
transduction of neonatal umbilical cord blood lymphocytes, the proliferation,
intracellular Ca++ concentration and phospholipase C γ1(PLCγ1)
expression of lymphocytes induced by anti-CD3 antibodies were detected.
Methods: 3H-Thymidine
incorporation was used to analyze the proliferative response;
fluorospectrophotometer was used to detect intracellular Ca++ concentration
and Western blot techque was performed to assess PLCγ1
expression.
Results: The
proliferation and intracellular Ca++ concentration of neonatal
umbilical cord blood lymphocytes induced by anti-CD3 antibodies was
increased, but the proliferation was significantly lower and intracellular
Ca++ concentration was slightly lower than that of adult. And the
PLCγ1 expression of neonatal umbilical cord blood
lymphocytes induced by anti-CD3 antibodies was markedly lower than that of
the adult.
Conclusions: The dysfunction mechanism of the signaling
transduction of neonatal umbilical cord blood lymphocytes maybe relate to
lower PLCγ1 expression.