THE EXPRESSION OF BRAIN-DERIVED NEUROTROPHIC
FACTOR (BDNF) IN NEONATAL RATS WITH HYPOXIC-ISCHEMIC BRAIN DAMAGE
Wang Q, Chen C, Liu DL ,Chen L, Yang Y
Children¡¯s
Hospital, Fudan University, Shanghai, China
Objective: To explore the role of
BDNF in hypoxic-ischemic brain damage (HIBD).
Methods: HIBD was produced by a
combination of unilateral carotid artery ligation and transient inhalation
hypoxia (8% O2). Fifty-four neonatal rats (7 day old) were
randomly divided into two groups: normal control group (n=6) and HIBD
group.The later was futher divided into eight groups:3, 6, 12, 24, 48, 72,
96, 120 hour after
HIBD.In
this study, in situ hybrization was used to observe the expression of BDNF
mRNA in the different regions at each time point. Levels of BDNF mRNA were
semiquantified by computerized image analysis using image software.
Results: The level of BDNF mRNA
increased from 6 hour and reached maximal levels on 48-72 hour after
insult. By day 5,the content of BDNF mRNA returned to control levels. BDNF
mRNA expression levels in resistant region (dentate granule) is higher than
that in susceptible regions (cortex). No significant difference between the
unligation region of HIBD group and control group (P>0.5).
Conclusions: The study showed that
endogenuous BDNF can protect neurons from HIBD.