BCL-2 AND BAX EXPRESSION AND ITS CLINICAL SIGNIFICANCE IN ACUTE
LEUKEMIA
Li Ge, Liao Qingkui, Fu Renyi, et al.
Department of
Pediatrics, Sichuan Provincial People¡¯s Hospital, Chengdu, China
Abstract:
Objective To investigate the expression, correlation and clinical
significance of bcl-2 and bax genes in AL.
Methods: The
semi-quantivative reverse transcription polymerase chain reaction (RT-PCR)
and Southern blot were used to the level and ratio of bcl-2 and bax mRNA in
32 AL cases.
Results: The
bcl-2 mRNA expression of the bone marrow cells was remarkably higher than
that of the control group (P<0.01). After the treatment, the bcl-2 mRNA
was obviously lower than that of of the control group (P>0.01), but
there was no difference in bax-mRNA in the both groups (P<0.05). The
analysis of the expression ratio of bcl-2 and bax mRNA showed the
nonremission (NR) rate in the high ratio group. Significantly higher than
that in lower ratio group (P<0.01).
Conclusion:
There is a relationship between AL and the abnormal expression of bcl-2 and
bax. bcl-2 and bax mRNA and their ratio may be served as a predictor for
the determination of AL prognosis and treatment.