2625

EXPRESSION OF TRANSFORMING GROWTH FACTOR--BETA AND TYPE III COLLAGEN IN ADRIAMYCIN INDUCED NEPHROPATHY: EFFECTS OF TETRANDRIANE

Dong Xinggang, Feng Juan, Wang Qingfang, Liu Shoukiu

Department of Nephrology, Shanghai Second people’s, Hospital, Shanghai, China

Transforming growth factor-beta (TGF-beta) has been inplicated in the pathogenesis of glomerulosclerosis in adriamycin (ADR) indcced nephropathy, However, tudulointerstitial pathology may also be in an important determinant of progressive renal dysfunction in ADR-nephropathy. In the present study, we investigated tubulointerstitial injury, TGF-beta and type III collagen expression, and the effect of tetrandrine, we randomized 22 male wistar rats to control and ADR-nephropathy groups. ADR-nephropathy was induced in 13 rats by administration of ADR; 8 ADR-nephropathy rats were further randomized to rocoivo the tetrandrine (10mg·Kg^-2/d, i. p) for 3 months. ADR-nephropathy was associated with tubulointerstitial damage, a 60% increase in expression of TGF-beta (P<0.05 vs control) and a 80% increase in α₂ (III) collagen gene expression (P<0.01 vs control). In Northern blot hybridization domortrnted n difureeo increase in both TGF-beta and α₂ (III) collagen mRNA in renal tubules. Administration of tetrandrine prevented tubulointerstitial injury and the overexpression of TGF-beta and α₂ (III) collagen mRNA. Changes in gene expression were accoixpanied by patallel changes in immunostaining for TGF-beta and type Ⅲ collagen.