THE EFFECTS OF NF-кB ON
THE ENDOTHELIAL DAMAGE MEDIATED BY INFLAMMATORY CYTOKINES IN
KAWASAKIDISEASE
Fu JR1, Li CR1, Zhou YD2
1Children’s Hospital of Shenzhen, Shenzhen, China
2Children’s Hospital of Chongqing, Chongqing,
China.
Objective: To further explore the
effects of nuclear factor-кB (NF-кB) on the endothelial damage mediated by
inflammatory cytokines in kawasaki diseae (KD).
Methods: Human umbilical vein
endothelial cells were cultured in vitro and the production of tumor
necrosis factor-alpha (TNF-α)、interleukin-1
beta(IL-1β)and interleukin-6
(IL-6) were measured by enzyme-linked immunosorbent assay (ELISA).
The proportion of apoptotic cells in endothelial cells induced by the
supernatants of peripheral blood mononuclear cells (PBMCs) was detected by
Annexin V/PI doublestaining. Electrophoretic mobility shift assay (EMSA)
was used to detect the activity of nuclear factor-кB
(NF-кB).
Results: The level of TNF-α、IL-1β、IL-6 were markedly
increased in KD patients and the proportion of apoptotic cells
in endothelial cells induced by the cultured supernatants of PBMCs was
markedly elevated (38.45±7.80%) compared to 2.87±0.76% in the control subjects. The apoptosis inducted by
the cultured supernatants of PBMCs could be reversed, to some degree,
through alone anti-TNF-α、IL-1β、IL-6
monoclonal antibody (McAb) or combining together. The activity of NF-кB in the activated PBMCs in KD patients was distinctly
increased and SN50, the blockade of NF-кB, could
significantly inhibit both the production of TNF-α、IL-1β、IL-6 and the apoptosis of endothelial cells induced by
the cultured supernatants of PBMC in KD patients.
Conclusion: NF-кB,
which can trigger the transcription of inflammatory cytokines such as TNF-α、IL-1β、IL-6, play an important
role in endothelial damage of KD.