TO ADOPT FINGER BLOOD
MEASURING SURFACE ANTIBODY OF RBC FOR THE DIAGNOSIS CHILDREN’S AIHA
Li Yun, Yi Hongling,
He Xiangling, Liang Mo, Tang Xuezhuan
Pediatrics
Department of Hunan People’s Hospital, Changsha, China
Objective:
Autoimmune hemolytic anemia (AIHA) can be
demonstrated by direct coombs’ test with venous blood of defibrinogen in
our laboratory before, but venous blood is hard to obtain and not suitable
for children. The study is for exploring methodology to measure surface
antibody of red blood cell (RBC) with minim finger blood for diagnosis of
children’s AIHA.
Methods: In a case 40ul blood was got from finger
capillary, with 10ml normal solution in a centriguge tube. The blood has
been washed three times and made into 2%RBC solution. Then direct
coombs’test can be used for examining surface antibody on RBC. In thirty
cases the defibrinated blood is compared with minim finger blood by two
methods.
Results: For five years, thirteen patients with AIHA
have been found using by the test of minim finger blood. The direct coombs’
tests were positive in all cases. Seven of them had positive result with
univalent antibody IgG; one IgG+C3; three IgG+IgM+C3; two IgM+C3. All the
cases accord with clinical diagnosis of AIHA. The results by two methods
are consistent with each other, in the four positive cases. In twenty-six
negative results cases, There was a false positive (3.85%)of univalent
antibody IgM in minim finger blood method. There were two false positive
(7.69%)of IgM in defibrinated blood. There was no statistics difference
(p>0.05) between the two methods. After the two different blood sources
are washed three times by analyzing protein of supernatant fluid after
centrifuging there was no statistics difference (p>0.05).
Conclusion: The study
of minim finger blood measuring RBC surface antibody suggests that AIHA
diagnosis can be made by this method. Compared with that of defibrinated
blood, the result is reliable, less blood consuming and convenient for
reexamining conveniently.