2677

GENE EXPRESSION OF MMP-3 IN PULMONARY VASCULARREMODELING OF RATS WITH PULMONARY HYPERTENSION INDUCED BY MONOCROTALINE

Wang DW, Qin YM

Nanjing Children's Hospital, Nanjing, China

Objective: To explore the etiology of pulmonary hypertension with relation to the pulmonary expression of metalloprotease3 (MMP-3).

Methods: The model of rat with pulmonary hypertension was induced by monocrotaline (MCT) subcutaneous injection and the pulmonary arterial pressure was measured by cathetrization and the pulmonary MMP-3mRNA expression levels were determined by RT-PCR The contents of hydroxyproline in main pulmonary artery were tested by using chromometry.

Results: The pulmonary arterial pressure was increased significantly on day 21 after monocrotaline injected and come to the peak on day 28 in the study The expression level of MMP-3 m RNA in pulmonary was increased significantly on day 7 and then come down gradually on day 14、21、28.The content of hydroxyproline n main pulmonary artery was decreased on day 14 compared with control then was increased on day 21、28.

Conclusion: MMP-3 over-expression involved in the development of pulmonary hypertension and might play the role of target during earlier stage of pulmonary arterial remodeling.