CHANGES OF THE S-100
PROTEIN IN EPILEPTIE SEIZURE INDUCED BY PTZ ON THE HIPPOEAMPAL IN RATS
Shi Rongfu, Tang
Hongxia, Sun Suzhen
No 133, South Jianhua
Street, Shijiazhuang, China
Objective: To explore the S-100
protein changes of epileptic seizure induced by pentylenetetrazol (PTZ) on
the hippocampal in rats reflect the brain damage especially for the
astyocytes in the epileptic seizure. It may provide a new way to control
the epilepsy.
Method: To select 42 SD rats.
Age: 4-6w, weight: 220g-250g. Distribute the rats into three groups as
control epilepsy and status epilepsy randomly. Epilepsy is made by
different dose of PTZ through i.p. and pick the brain after seizure at 6h,
12h, 24h, 72h respectively. Finally, defect the S-100 protein immunoreact positive
cell in the hippocampal through immunohisto-chemistry.
Result: In epilepsy group: S-100
protein IR positive cells begin to rise at 12h and reach the peak at 24th.
At 72h, it began its reduction. The changes have obvious difference when
compare with control group. In status epilepsy group, either 12h or 24h
have obvious and more significant than epilepsy group.
Conclusion:
In epileptic rats, the number of S-100 protein IR positive Cell rise
obviously. The changes have the relation to the last time and degree of
seizure. So the S-200 protein can be regard as a sensitive and special
indicator to reflect the brain damage, especially the astrocyte damage in
epilepsy seizure.
Key
Words: S-100 protein, astrocyte, epilepsy, rat, hippocampal