ALTERATIONS OF GENE EXPRESSION OF SR Ca­­2+_ TRANSFER

PROTEINS IN INFANTS WITH CONGESTIVE HEART FAILURE

Yin W, Zheng X-Q, Deng S-Z

Department of Cardiology, Wuhan Children’s Hospital, Wuhan, China

 

Objective: To investigate changes of mRNA expression levels of calcium-adenosin triphosphatase and ryanodine receptor in infants with congestive heart failure (CHF); To explore the molecular mechanism of CHF in infants.

Methods: By reverse transcription-polymerase chain reaction (RT-PCR), we measured expression levels of SR Ca2+-ATPase mRNA and RyR mRNA in the right atrial tissue from fourty eight children (M 28, F20) with congenital heart defect betreen the ages of 6 months and 3 years. They were divided into three groups with CHF (group A: moderate to severe CHF; group B: mild CHF; group C: No CHF).

Results: The level of SR Ca2+-ATPase mRNA in group A was significantly lower than group B and C (P<0.01). The level in group B was mildly higher than group C, but without statistical significance (P>0.05); The level of SR RyR mRNA in group A was significantly lower than group B (P<0.05) and group B also siginificantly lower than group C (P<0.05). Meanwhile, the RyR mRNA expression level was linear related negatively with the CHF severity (Ross mark) ( r = -0.36, P<0.05).

Conclusion: The down-regulation of cardiac SR Ca2+-ATPase mRNA and RyR mRNA expression maybe the key fectors of development of CHF in infants.

 

 
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